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bio dot microfiltration blotting device  (Bio-Rad)


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    Structured Review

    Bio-Rad bio dot microfiltration blotting device
    Bio Dot Microfiltration Blotting Device, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1624 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bio+dot+microfiltration+blotting+device/Bio-Dot+Microfiltration+Apparatus/pmc12443267-61-19-23
    Average 99 stars, based on 1624 article reviews
    bio dot microfiltration blotting device - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Pore Size:

    Article Title: The Glycolytic Enzyme Triosephosphate Isomerase of Trichomonas vaginalis Is a Surface-Associated Protein Induced by Glucose That Functions as a Laminin- and Fibronectin-Binding Protein
    Article Snippet: .. Different amounts (0, 0.1, 0.3, 0.5, 1.0, 2.0, and 4.0 μg) of Tv TIM1r and Tv TIM2r were transferred onto NC membranes (0.2-μm pore size; Bio-Rad) using a Bio-Dot microfiltration blotting device (Bio-Rad). ..

    Modification:

    Article Title: Spreading of Alzheimer’s disease inflammatory signaling through soluble micro-RNA
    Article Snippet: .. LED-Northern dot-blot analysis was performed using a modified Bio-Dot microfiltration blotting device (LED= LNA, EDC, and DIG; l ocked nucleic acids – E DC – d igoxigenin; detection limit=0.05 fM of a single miRNA species; apparatus #170-6545, BioRad Life Science Research, Hercules, California, USA) [ 10 – 18 , 23 ]. .. LED-Northern dot blots are a significant advancement over classical Northern blotting techniques because they utilize LNA-stabilized miRNAs or anti-miRNAs(AMs) covalently linked to a nylon-based membrane matrix (using EDC) and are probed using DIG-labeled small RNAs with fluorescent reporters [ 19 – 22 ]. miRNA and/or AMs, as locked nucleic acid (LNA) oligonucleotides, included a control miRNA-183 (5′-TATGGCACTGGTAGAATTCACT-3′), anti-miRNA-155 (AM-155; 5′-AATTACGATTAGCACTATCCCCA-3′), and a scrambled control AM-155 (AM-155sc; 5′-TTAACATTAGACGATATCCCACC-3′) were purchased from Applied Biosystems/Ambion, Austin, Texas, USA or Exiqon Inc., Woburn, Massachusetts, USA [ 11 , 16 ], and were used at a 5–20 nM ambient concentration that was replenished at every change of ABM (see above; Lonza, Basel, Switzerland) for a total treatment time of 12 and 36 h after the induction of TNF-α + Aβ42 peptide. miRNA-146a and AM-146a sequences and their usage have been described previously [ 12 – 17 ].

    Article Title: Deficiency in the Ubiquitin Conjugating Enzyme UBE2A in Alzheimer’s Disease (AD) is Linked to Deficits in a Natural Circular miRNA-7 Sponge (circRNA; ciRS-7)
    Article Snippet: Samples were analyzed on miRNA arrays employing μParaflo® Microfluidic Biochip Technologies that interrogate the abundance and speciation of ~2650 human miRNAs (LC Sciences, Houston, TX, USA). .. LED-Northern dot-blot analysis was performed using a modified Bio-Dot microfiltration blotting device (LED = LNA, EDAC and DIG; LNA = locked nucleic acids; EDAC = 1-ethyl-3-3-dimethylaminopropyl carbodiimide; DIG = digoxigenin; detection limit = 0.05 fM of a single miRNA species; apparatus #170-6545, BioRad Life Science Research, Hercules, CA, USA) [ , , , , , ]. ..

    Article Title: Regulation of TREM2 expression by an NF-?B-sensitive miRNA-34a
    Article Snippet: .. LED-northern dot blot analysis was performed using a modified Bio-Dot microfiltration blotting device (detection limit=0.05 fM for a single miRNA species, apparatus #170-6545; BioRad Life Science Research, Hercules, California, USA) [ 9 , 11 , 12 , 19 ]. ..

    Membrane:

    Article Title: Translocator protein (TSPO) analysis in saliva of adults with oral mucosal lesions: A preliminary study
    Article Snippet: .. Samples were spotted by duplicate directly onto a nitrocellulose membrane (AmershanTM ProtanTM 0.45 μm, catalogue (cat.) #10600002) using a Bio-Dot® microfiltration blotting device (Bio Rad laboratories, Inc., cat. #1706545). ..

    Dot Blot:

    Article Title: Regulation of TREM2 expression by an NF-?B-sensitive miRNA-34a
    Article Snippet: .. LED-northern dot blot analysis was performed using a modified Bio-Dot microfiltration blotting device (detection limit=0.05 fM for a single miRNA species, apparatus #170-6545; BioRad Life Science Research, Hercules, California, USA) [ 9 , 11 , 12 , 19 ]. ..



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